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RNA-guided <i>As</i> Cas12a- and <i>Sp</i> Cas9-catalyzed knockout and homology directed repair of the <i>omega-1</i> locus of the human blood fluke, <i>Schistosoma mansoni</i>

2021-11-16

Abstract excerpt

We compared the efficiency of gene knockout (KO) and precision of insertion (knock-in, KI) of the RNA-guided As Cas12a nuclease of Acidaminococcus sp. with that of Sp Cas9 from Streptococcus pyogenes , aiming to enhance the functional genomics toolkit for Schistosoma mansoni . Programmed DNA cleavages catalyzed by Cas12a and Cas9 result in staggered and blunt ended strand breaks, respectively. TTTV, the optim...

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Literature Corpus work
9b004760-e318-5eb1-9973-3c9742303ebc
DOI
10.1101/2021.11.15.468743
Open publication

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RNA-guided <i>As</i> Cas12a- and <i>Sp</i> Cas9-catalyzed knockout and homology directed repair of the <i>omega-1</i> locus of the human blood fluke, <i>Schistosoma mansoni</i>DOI 10.1101/2021.11.15.468743
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