Back to search

Article

ENHANCED EFFICIENCY OF RNA-GUIDED CAS12a VERSUS CAS9 TRANSGENE KNOCK-IN AND ACTIVITY AT A <i>SCHISTOSOMA MANSONI</i> GENOME SAFE HARBOR

2023-09-12

Abstract excerpt

<h4>ABSTRACT</h4> Recently, we reported programmed Cas9 mediated insertion of a reporter gene into a gene safe harbor site, GSH1, of Schistosoma mansoni via homology-directed repair (HDR) using overlapping guide RNAs. Here, we report efficient and precise CRISPR/Cas12a-mediated homology directed insertion (knockin, KI) of a 5’ C6-PEG10-modified double-stranded transgene bearing microhomology arms, 50 nt in lengt...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
8f1b2d9e-d78c-585f-b260-ffd3bca06fac
DOI
10.1101/2023.09.12.557428
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
ENHANCED EFFICIENCY OF RNA-GUIDED CAS12a VERSUS CAS9 TRANSGENE KNOCK-IN AND ACTIVITY AT A <i>SCHISTOSOMA MANSONI</i> GENOME SAFE HARBORDOI 10.1101/2023.09.12.557428
Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, <i>Schistosoma mansoni</i>Semantic proximity
RNA-guided <i>As</i> Cas12a- and <i>Sp</i> Cas9-catalyzed knockout and homology directed repair of the <i>omega-1</i> locus of the human blood fluke, <i>Schistosoma mansoni</i>Semantic proximity
RNA-Guided AsCas12a- and SpCas9-Catalyzed Knockout and Homology Directed Repair of the Omega-1 Locus of the Human Blood Fluke, Schistosoma mansoni.Semantic proximity
CRISPR/Cas9-mediated genome editing of <i>Schistosoma mansoni</i> acetylcholinesteraseSemantic proximity
Rapid and visual detection of <i>Schistosoma japonicum</i> circulating nucleic acids by CRISPR-Cas12aSemantic proximity
Large CRISPR-Cas-induced deletions in the oxamniquine resistance locus of the human parasite Schistosoma mansoniSemantic proximity
Large CRISPR-Cas-induced deletions in the oxamniquine resistance locus of the human parasite <i>Schistosoma mansoni</i>Semantic proximity
Programmed genome editing of the omega-1 ribonuclease 1 of the blood fluke, <i>Schistosoma mansoni</i>Semantic proximity
CRISPR-assisted test for Schistosoma haematobiumSemantic proximity
Efficient Editing of Malaria Parasite Genome Using the CRISPR/Cas9 SystemSemantic proximity
Ribozyme-Mediated, Multiplex CRISPR Gene Editing and CRISPRi in <i>Plasmodium yoelii</i>Semantic proximity
CRISPR-mediated knock-in of transgenes into the malaria vector <i>Anopheles funestus</i>Semantic proximity
Select a neighboring publication to make it the new centre.