Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

RNA-Guided AsCas12a- and SpCas9-Catalyzed Knockout and Homology Directed Repair of the Omega-1 Locus of the Human Blood Fluke, Schistosoma mansoni.

International journal of molecular sciences - 6 Jan 2022

Ittiprasert Wannaporn, Chatupheeraphat Chawalit, Mann Victoria H, Li Wenhui, Miller André, Ogunbayo Taiwo, Tran Kenny, Alrefaei Yousef N, Mentink-Kane Margaret, Brindley Paul J

Abstract excerpt

The efficiency of the RNA-guided AsCas12a nuclease of Acidaminococcus sp. was compared with SpCas9 from Streptococcus pyogenes, for functional genomics in Schistosoma mansoni. We deployed optimized conditions for the ratio of guide RNAs to the nuclease, donor templates, and electroporation parameters, to target a key schistosome enzyme termed omega-1. Programmed cleavages catalyzed by Cas12a and Cas9 resulted in...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Gene Knockout Techniques
Share to Genes, Protozoan

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Oligonucleotides

Share to Recombinational DNA Repair

Share to Reference Standards

Share to Ribonucleases

Share to RNA, Guide, CRISPR-Cas Systems

Share to Schistosoma mansoni

Source

Open in PubMed Open full text

Identifiers

PMID: 35054816
DOI: 10.3390/ijms23020631
PMCID: PMC8775552

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

RNA-Guided AsCas12a- and SpCas9-Catalyzed Knockout and Homology Directed Repair of the Omega-1 Locus of the Human Blood Fluke, Schistosoma mansoni.PMID 35054816Read ↗

RNA-guided <i>As</i> Cas12a- and <i>Sp</i> Cas9-catalyzed knockout and homology directed repair of the <i>omega-1</i> locus of the human blood fluke, <i>Schistosoma mansoni</i>Semantic proximity Read ↗

ENHANCED EFFICIENCY OF RNA-GUIDED CAS12a VERSUS CAS9 TRANSGENE KNOCK-IN AND ACTIVITY AT A <i>SCHISTOSOMA MANSONI</i> GENOME SAFE HARBORSemantic proximity Read ↗

Programmed genome editing of the omega-1 ribonuclease 1 of the blood fluke, <i>Schistosoma mansoni</i>Semantic proximity Read ↗

Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, <i>Schistosoma mansoni</i>Semantic proximity Read ↗

Large CRISPR-Cas-induced deletions in the oxamniquine resistance locus of the human parasite Schistosoma mansoniSemantic proximity Read ↗

Large CRISPR-Cas-induced deletions in the oxamniquine resistance locus of the human parasite <i>Schistosoma mansoni</i>Semantic proximity Read ↗

CRISPR/Cas9-mediated genome editing of <i>Schistosoma mansoni</i> acetylcholinesteraseSemantic proximity Read ↗

Efficient Editing of Malaria Parasite Genome Using the CRISPR/Cas9 SystemSemantic proximity Read ↗

A Type II-B Cas9 nuclease with minimized off-targets and reduced chromosomal translocations in vivo.Semantic proximity Read ↗

Prime editing outperforms homology-directed repair as a tool for CRISPR-mediated variant knock-in in zebrafishSemantic proximity Read ↗

High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairsSemantic proximity Read ↗

Genome-engineering with CRISPR-Cas9 in the mosquito <i>Aedes aegypti</i>Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.