Article

CRISPR/Cas9-mediated genome editing of <i>Schistosoma mansoni</i> acetylcholinesterase

2020-07-09

Abstract excerpt

CRISPR/Cas9-mediated genome editing shows cogent potential for the genetic modification of helminth parasites. Here we report successful gene knock-in (KI) into the genome of the egg of Schistosoma mansoni by combining CRISPR/Cas9 with single-stranded oligodeoxynucleotides (ssODNs). We edited the acetylcholinesterase (AChE) gene of S. mansoni targeting two guide RNAs (gRNAs), X5 and X7, located on exon 5 and exo...

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Literature Corpus work: be22b929-bbc8-5fdc-a625-4833dc640d7a
DOI: 10.1101/2020.07.08.190694

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