Article
Engineered FnCas12a with enhanced activity through directional evolution in human cells.
The Journal of biological chemistry - 1 Jan 2000
Liu Xiexie, Liu Xiaoyu, Zhou Chenchen, Lv Jineng, He Xiubin, Liu Yuanyuan, Xie Haihua, Wang Bang, Lv Xiujuan, Tang Lianchao, Li Mingchun, Liu Changbao, Zhao Junzhao, Liu Yong, Song Zongming, Gu Feng
Abstract excerpt
Clustered regularly interspaced short palindromic repeat-Cas12a has been harnessed to manipulate the human genome; however, low cleavage efficiency and stringent protospacer adjacent motif hinder the use of Cas12a-based therapy and applications. Here, we have described a directional evolving and screening system in human cells to identify novel FnCas12a variants with high activity. By using this system, we...
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