Article
Engineered Un1Cas12f1 for multiplex genome editing with enhanced activity and targeting scope.
Nature communications - 19 Feb 2026
Huo Yanan, Mei Jiale, Zhang Dan, Yan Bing, Zhang Dexin, Dong Chao, Yin Shuming, Liu Meizhen, Wang Xinyan, Chen Dan, Guan Yuting, Song Gaojie, Du Bing, Wang Yongming, Zheng Zongli, Liu Hong, Li Dali, Yang Lei, Wang Liren
Abstract excerpt
The compact CRISPR-Cas12f system is promising for AAV-delivered gene therapy, but its application has been constrained by restrictive PAM recognition (e.g., TTTR) and suboptimal editing efficiency. Through bacterial library screening and mammalian cell validation, we engineer evoCas12f, an optimized variant incorporating five key mutations, that dramatically expands PAM recognition to NTNR/NYTR. This advancement...
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