Article

The human Origin Recognition Complex is essential for pre-RC assembly, mitosis and maintenance of nuclear structure

2020-08-06

Abstract excerpt

The Origin Recognition Complex (ORC) cooperates with CDC6, MCM2-7, and CDT1 to form pre- RC complexes at origins of DNA replication. Here we report tiling-sgRNA CRISPR screens that show that each subunit of ORC and CDC6 are essential in human cells. Using an auxin-inducible degradation system, stable cell lines were created that ablate ORC2 rapidly, revealing multiple cell division cycle phenotypes. The primary de...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 95d09980-203d-5445-bf72-1e26a38de28b
DOI: 10.1101/2020.08.06.240358

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

The human Origin Recognition Complex is essential for pre-RC assembly, mitosis and maintenance of nuclear structureDOI 10.1101/2020.08.06.240358Read

A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsSemantic proximity Read

Two subunits of human ORC are dispensable for DNA replication and proliferationSemantic proximity Read

A Meier-Gorlin Syndrome Mutation in Orc4 Causes Tissue-Specific DNA Replication Defects in <i>Drosophila melanogaster</i>Semantic proximity Read

Tissue-Specific DNA Replication Defects in Drosophila melanogaster Caused by a Meier-Gorlin Syndrome Mutation in Orc4.Semantic proximity Read

A WD-repeat protein stabilizes ORC binding to chromatin.Semantic proximity Read

Human CCR4-NOT globally regulates gene expression and is a novel silencer of retrotransposon activationSemantic proximity Read

Orc6 involved in DNA replication, chromosome segregation, and cytokinesis.Semantic proximity Read

The origin recognition complex is dispensable for endoreplication in Drosophila.Semantic proximity Read

Human CCR4-NOT suppresses pervasive transcription and retrotransposable elementsSemantic proximity Read

Regulation of epigenetics and chromosome structure by human ORC2Semantic proximity Read

Mitosis without DNA replication in mammalian somatic cellsSemantic proximity Read

The ORC ubiquitin ligase OBI1 promotes DNA replication origin firingSemantic proximity Read