Article

The effects of length and sequence of gRNA on Cas13b and Cas13d activity in vitro and in vivo

2022-10-25

Abstract excerpt

Cas13 are the only CRISPR/Cas systems found so far, which target RNA strand while preserving chromosomal integrity. Cas13b or Cas13d cleaves RNA by the crRNA guidance. However, the effect of the characteristics of the spacer sequences, such as the length and sequence preference, on the activity of Cas13b and Cas13d remains unclear. Our study shows that neither Cas13b nor Cas13d has a particular preference for the...

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Literature Corpus work: a841a6c6-dce0-5c25-8351-a38feab4ea05
DOI: 10.22541/au.166668845.54686270/v1

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