Article
Characteristics of mutations generated through digestion with restriction enzyme and ligation in plasmid DNA.
Environmental and molecular mutagenesis - 1 Jan 2001
Nakamura S, Ikehata H, Ono T
Abstract excerpt
Recently, the use of restriction enzymes has been extended to studies in which rare events such as mutation and mistakes in DNA repair are examined. In these studies, the specificity of restriction enzymes becomes critical. To clarify the nature of the rare unexpected events occurring in the process of cutting of DNA with restriction enzymes then ligating it, we studied the molecular characteristics of unexpected...
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