Article
Site-directed mutagenesis of double-stranded DNA by the polymerase chain reaction.
Gene - 30 Dec 1994
Weiner M P, Costa G L, Schoettlin W, Cline J, Mathur E, Bauer J C
Abstract excerpt
We have developed a facile procedure for rapid PCR-based site-directed mutagenesis of double-stranded DNA. Increasing the initial template concentration and decreasing the PCR cycles to 5-10 allows us to reduce the rate of undesired second-site mutations and dramatically increase the time savings...
Topics
- Base Sequence
- DNA
- DNA Primers
- Deoxyribonucleases, Type II Site-Specific
- Escherichia coli
- Escherichia coli Proteins
- Indicators and Reagents
- Methyltransferases
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Polymerase Chain Reaction
- Site-Specific DNA-Methyltransferase (Adenine-Specific)