Article

An integrated enzymatic and computational pipeline for quantifying off-target base-editing

2025-08-26

Abstract excerpt

DNA base editing is increasingly used for human genetic modification, but methods for monitoring off-target editing are nascent. Here we present a simple model-independent workflow for identifying sites of off-target base-editing in relevant cell types on a genome-wide level. We report that sites of off-target editing by the ABE8e editor could be identified using an ABE8e derivative with restored DSB cleavage acti...

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Literature Corpus work: 8e9e1c09-52f5-5822-bce3-b6a97caf1839
DOI: 10.1101/2025.08.26.667396

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