CRISPR base editing applications for identifying cancer-driving mutations.

Abstract excerpt

CRISPR base editing technology is a promising genome editing tool as (i) it does not require a DNA template to introduce mutations and (ii) it avoids creating DNA double-strand breaks, which can lead to unintended chromosomal alterations or elicit an unwanted DNA damage response. Given many cancers originate from point mutations in cancer-driving genes, the application of base editing for either modelling tumour...

Topics

• Animals
• CRISPR-Cas Systems
• Cell Transformation, Neoplastic
• Clustered Regularly Interspaced Short Palindromic Repeats
• DNA Mutational Analysis
• Gene Editing
• Genes, Neoplasm
• Humans
• Mutation
• Neoplasms
• Oncogenes