Article

Directed Evolution of Adenine Base Editors with Increased Activity and Therapeutic Application

2020-03-16

Abstract excerpt

The foundational adenine base editors ( e.g. ABE7.10) enable programmable C•G to T•A point mutations but editing efficiencies can be low at challenging loci in primary human cells. Here we further evolve ABE7.10 using a library of adenosine deaminase variants to create ABE8s. At NGG PAM sites, ABE8s result in ∼1.5x higher editing at protospacer positions A5-A7 and ∼3.2x higher editing at positions A3-A4 and A8-A1...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 160076c8-df66-5a68-a580-aa242884de2a
DOI: 10.1101/2020.03.13.990630

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Directed Evolution of Adenine Base Editors with Increased Activity and Therapeutic ApplicationDOI 10.1101/2020.03.13.990630Read

Directed evolution of adenine base editors with increased activity and therapeutic application.Semantic proximity Read

Human cell based directed evolution of adenine base editors with improved efficiency.Semantic proximity Read

Human cells based directed evolution of adenine base editors with improved efficiencySemantic proximity Read

Engineering a precise adenine base editor with minimal bystander editing.Semantic proximity Read

Therapeutic adenine base editing of human hematopoietic stem cellsSemantic proximity Read

Engineering precise adenine base editor with infinitesimal rates of bystander mutations and off-target editingSemantic proximity Read

Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity.Semantic proximity Read

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage.Semantic proximity Read

Triple base editor catalyzes saturation mutation of adenine, cytidine, and guanine.Semantic proximity Read

Adenine transversion editors enable precise, efficient A•T-to-C•G base editing in mammalian cells and embryos.Semantic proximity Read

Both A-to-G and C-to-T base editing in human cells with dual base editor (A&C-BEmax)Semantic proximity Read

Analysis and minimization of cellular RNA editing by DNA adenine base editorsSemantic proximity Read