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Article

Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID).

Current protocols in cell biology - 2 Sept 2014

Nishimura Kohei, Kanemaki Masato T

Abstract excerpt

The auxin-inducible degron (AID) system allows the rapid and reversible proteolysis of proteins of interest, and enables the generation of conditional mutants of budding yeast. The construction of budding yeast AID mutants is simple, and the effect of depletion of essential proteins on proliferation can be confirmed by analyzing their phenotype. In this protocol, we describe a procedure to generate AID mutants of...

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PMID: 25181302
DOI: 10.1002/0471143030.cb2009s64

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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