Article

Highly efficient generation of GGTA1 knockout pigs using a combination of TALEN mRNA and magnetic beads with somatic cell nuclear transfer

2016-07-01

Abstract excerpt

The transcription activator-like effector nuclease (TALEN) technique combined with the somatic cell nuclear transfer (SCNT) method has been successfully applied for creating genetically modified pigs. However, methods for isolating cells with biallelic indels requires further improvement because of the relatively low enrichment efficiency of mutated somatic cells. Moreover, little is known regarding the off-target...

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Literature Corpus work: 8cc0b43e-9cee-587d-bcdc-aa7ddd605c70
DOI: 10.1016/s2095-3119(16)61347-3

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