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Article

Generation of α1,3-galactosyltransferase and cytidine monophospho-N-acetylneuraminic acid hydroxylase gene double-knockout pigs.

The Journal of reproduction and development - 1 Jan 2015

Miyagawa Shuji, Matsunari Hitomi, Watanabe Masahito, Nakano Kazuaki, Umeyama Kazuhiro, Sakai Rieko, Takayanagi Shuko, Takeishi Toki, Fukuda Tooru, Yashima Sayaka, Maeda Akira, Eguchi Hiroshi, Okuyama Hiroomi, Nagaya Masaki, Nagashima Hiroshi

Abstract excerpt

Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) are new tools for producing gene knockout (KO) animals. The current study reports produced genetically modified pigs, in which two endogenous genes were knocked out. Porcine fibroblast cell lines were derived from homozygous α1,3-galactosyltransferase (GalT) KO pigs. These cells were subjected to an additional KO for the...

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Identifiers

PMID: 26227017
DOI: 10.1262/jrd.2015-058
PMCID: PMC4623151

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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