Article
Generation of GGTA1 biallelic knockout pigs via zinc-finger nucleases and somatic cell nuclear transfer.
Science China. Life sciences - 1 Feb 2014
Bao Lei, Chen HaiDe, Jong UiMyong, Rim CholHo, Li WenLing, Lin XiJuan, Zhang Dan, Luo Qiong, Cui Chun, Huang HeFeng, Zhang Yan, Xiao Lei, Fu ZhiXin
Abstract excerpt
Genetically modified pigs are valuable models of human disease and donors of xenotransplanted organs. Conventional gene targeting in pig somatic cells is extremely inefficient. Zinc-finger nuclease (ZFN) technology has been shown to be a powerful tool for efficiently inducing mutations in the genome. However, ZFN-mediated targeting in pigs has rarely been achieved. Here, we used ZFNs to knock out the porcine α-1,...
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