Article

SapTrap assembly of <i>C. elegans</i> MosSCI transgene vectors

2019-10-17

Abstract excerpt

The Mos1-mediated Single-Copy Insertion (MosSCI) method is widely used to establish stable Caenorhabditis elegans transgenic strains. Cloning MosSCI targeting plasmids can be cumbersome because it requires assembling multiple genetic elements including a promoter, a 3’UTR and gene fragments. Recently, Schwartz and Jorgensen developed the SapTrap method for the one-step assembly of plasmids containing components o...

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Literature Corpus work: 8c849a13-03ef-590f-9624-6efd0ed81300
DOI: 10.1101/805507

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