Article

Rapid generation of <i>C. elegans</i> single copy transgenes combining RMCE and drug selection

2023-03-05

Abstract excerpt

I outline a streamlined method to create single copy large genomic insert transgenes using Recombination-Mediated Cassette Exchange (RMCE) that relies solely on drug selection yielding a homozygous fluorescent protein (FP) marked transgene in 3 generations (8 days) at high efficiency (>1 insertion per 2 injected P0 animals). Landing sites for this approach are available on four chromosomes in several configuration...

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Literature Corpus work: 283c5659-7af0-5a83-8e9a-eca23f0c5877
DOI: 10.1101/2023.03.05.531207

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