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Article

A Simple Apparatus for Individual C. elegans Culture.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020

Pittman William E, Sinha Drew B, Kinser Holly E, Patil Nisha S, Terry Eric S, Plutzer Isaac B, Hong Julia, Pincus Zachary

Abstract excerpt

We miniaturized standard, solid-phase C. elegans culture conditions to produce a system in which many isolated, individual C. elegans can be housed throughout their lives. This system, the "worm corral," is compatible with high-resolution brightfield and fluorescent microscopy, allowing imaging of fluorescent transgenes and morphological phenotypes from hatch until death. These culture devices can be constructed...

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PMID: 32410022
DOI: 10.1007/978-1-0716-0592-9_3

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