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Split-wrmScarlet and split-sfGFP: Tools for faster, easier fluorescent labeling of endogenous proteins in <i>Caenorhabditis elegans</i>

2020-07-03

Abstract excerpt

We create and share a new red fluorophore, along with a set of strains, reagents and protocols, to make it faster and easier to label endogenous C. elegans proteins with fluorescent tags. CRISPR-mediated fluorescent labeling of C. elegans proteins is an invaluable tool, but it is much more difficult to insert fluorophore-size DNA segments than it is to make small gene edits. In principle, high-affinity asymmetri...

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Literature Corpus work
b8f26af9-f2a9-5292-9986-ea1d88c9de4c
DOI
10.1101/2020.07.02.185249
Open publication

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