Article

MAGIK: A rapid and efficient method to create lineage-specific reporters in human pluripotent stem cells

2023-09-10

Abstract excerpt

<h4>Summary</h4> Precise insertion of a fluorescent protein into a lineage-specific gene in human pluripotent stem cells (hPSCs) presents challenges due to the low knockin efficiency and difficulties in selecting the correctly targeted cells. Here we introduce the ModRNA-based Activation for Gene Insertion and Knockin (MAGIK) approach to enhance knockin efficacy in hPSCs. MAGIK operates in two steps: first, it em...

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Literature Corpus work: 89ba8fcf-3bed-5744-9611-1ffcfff88101
DOI: 10.1101/2023.09.07.556774

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