Article

Combinatorial and Inducible CRISPRa/i Enables Canalized hiPSC Forward Programming and Iterative Refinement <i>via</i> Single-Cell Genomics

2026-06-01

Abstract excerpt

<h4>SUMMARY</h4> Synthetic gene-regulation logic is established in immortalized cell lines but remains largely aspirational in human induced pluripotent stem cells (hiPSCs) and derivatives. This gap constrains both mechanistic discovery and translational engineering in physiologically relevant models. We developed CIRI ( C ombinatorial I nducible C R ISPR in I PSCs), an isogenic, safe-harbor–engineered platf...

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Literature Corpus work: 40ce3150-ce76-5e31-91c2-1022853eb8e0
DOI: 10.64898/2026.05.31.729073

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