Article
Mechanism of translation of monocistronic and multicistronic human immunodeficiency virus type 1 mRNAs.
Molecular and cellular biology - 1 Jan 1992
Schwartz S, Felber B K, Pavlakis G N
Abstract excerpt
We have used a panel of cDNA clones expressing wild-type and mutant human immunodeficiency virus type 1 (HIV-1) mRNAs to study translation of these mRNAs in eucaryotic cells. The tat open reading frame (ORF) has a strong signal for translation initiation, while rev and vpu ORFs have weaker signals. The expression of downstream ORFs is inhibited in mRNAs that contain the tat ORF as the first ORF. In contrast,...
Topics
- Base Sequence
- DNA, Viral
- Gene Expression Regulation, Viral
- Genes, rev
- Genes, tat
- HIV-1
- Molecular Sequence Data
- Mutation
- Open Reading Frames
- Precipitin Tests
- Protein Biosynthesis