Article

Development of deaminase-free T-to-S base editor and C-to-G base editor by engineered human uracil DNA glycosylase

2024-01-01

Abstract excerpt

DNA base editors could enable direct editing of adenine (A), cytosine (C), or guanine (G), but there is no base editor for direct thymine (T) editing currently. Here, by fusing Cas9 nickase (nCas9) with engineered human uracil DNA glycosylase (UNG) variants, we developed a deaminase-free glycosylase-based thymine base editor (gTBE) with the ability of direct T editing. By several rounds of UNG mutagenesis via rati...

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Identifiers and source

Literature Corpus work: 2fbdb891-97e7-5690-bdb7-7a320999d235
DOI: 10.1101/2024.01.01.573809

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