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Single-stranded HDR templates with truncated Cas12a-binding sequences improve knock-in efficiencies in primary human T cells

19 May 2025

Abstract excerpt

CRISPR-Cas12a gene editing offers an alternative to Cas9-based methods, providing better targeting of AT-rich regions, simplified guide RNA manufacturing, and high specificity. However, the efficacy of donor-based editing is subject to various factors, with template format playing a crucial role. Currently, the predominant non-viral template format for homology-directed repair (HDR) after nuclease-induced DNA...

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PMID: 40520364
DOI: 10.1016/j.omtn.2025.102568

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