Article

Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturing

2021-09-04

Abstract excerpt

CRISPR-Cas9 offers unprecedented opportunities to modify genome sequences in primary human cells to study disease variants and reprogram cell functions for next-generation cellular therapies. CRISPR has several potential advantages over widely used retroviral vectors including: 1) site-specific transgene insertion via homology directed repair (HDR), and 2) reductions in the cost and complexity of genome modificati...

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Literature Corpus work: 049f0610-3c67-58d1-95b7-7ff38eeaab4b
DOI: 10.1101/2021.09.02.458799

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