Article

A Cas9 nanoparticle system with truncated Cas9 target sequences on DNA repair templates enhances genome targeting in diverse human immune cell types

2019-03-28

Abstract excerpt

<h4>ABSTRACT</h4> Virus-modified T cells are approved for cancer immunotherapy, but more versatile and precise genome modifications are needed for a wider range of adoptive cellular therapies 1–4 . We recently developed a non-viral CRISPR–Cas9 system for genomic site-specific integration of large DNA sequences in primary human T cells 5 . Here, we report two key improvements for efficiency and viability in an e...

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Literature Corpus work: aebe108c-8d70-5074-a122-1b9ac6a3f467
DOI: 10.1101/591719

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