Back to search

Article

Single-stranded HDR templates with truncated Cas12a binding sequences improve knock-in efficiencies in primary human T cells

2024-09-11

Abstract excerpt

Non-viral gene editing via CRISPR-Cas12a offers an alternative to Cas9-based methods, providing better targeting of AT-rich regions, simplified guide RNA manufacturing, and high specificity. However, the efficacy of editing outcomes is subject to various factors, with template format playing a crucial role. Currently, the predominant non-viral template format for inducing homology-directed repair (HDR) after nucle...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
4e3e4d75-f12e-5d65-85a5-572afba315a9
DOI
10.1101/2024.09.11.608426
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Single-stranded HDR templates with truncated Cas12a binding sequences improve knock-in efficiencies in primary human T cellsDOI 10.1101/2024.09.11.608426
Single-stranded HDR templates with truncated Cas12a-binding sequences improve knock-in efficiencies in primary human T cellsSemantic proximity
Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturingSemantic proximity
High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails.Semantic proximity
A Cas9 nanoparticle system with truncated Cas9 target sequences on DNA repair templates enhances genome targeting in diverse human immune cell typesSemantic proximity
Repurposing base editors for targeted knock-in and simultaneous knockouts to generate multiplex-edited allogeneic CAR T cells with minimal translocationsSemantic proximity
The CRISPR-Cas12a Platform for Accurate Genome Editing, Gene Disruption, and Efficient Transgene Integration in Human Immune CellsSemantic proximity
Protocol for Non-viral HDR-based CRISPR/Cas9 platform for small custom editing in primary T cellsSemantic proximity
CRISPR-Mediated Non-Viral Site-Specific Gene Integration and Expression in T Cells: Protocol and Application for T-Cell TherapySemantic proximity
Circular single-stranded DNA is a superior homology-directed repair donor template for efficient genome engineeringSemantic proximity
Dynamics and competition of CRISPR–Cas9 ribonucleoproteins and AAV donor-mediated NHEJ, MMEJ and HDR editingSemantic proximity
Combining different CRISPR nucleases for simultaneous knock-in and base editing prevents translocations in multiplex-edited CAR T cellsSemantic proximity
Engineering Tripartite Gene Editing Machinery for Highly Efficient Non-Viral Targeted Genome IntegrationSemantic proximity
Select a neighboring publication to make it the new centre.