Saturation mutagenesis of a predicted ancestral Syk-family kinase

Abstract excerpt

<h4>ABSTRACT</h4> Many tyrosine kinases cannot be expressed readily in E. coli , limiting facile production of these proteins for biochemical experiments. We used ancestral sequence reconstruction to generate a spleen tyrosine kinase (Syk) variant that can be expressed in bacteria and purified in soluble form, unlike the human members of this family (Syk and ZAP-70). The catalytic activity, substrate specificity...

Topics

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• Combinatorial Chemistry Techniques
• Gene Library
• Job Syndrome
• Molecular Docking Simulation
• Molecular Dynamics Simulation
• Mutation
• Protein Kinase Inhibitors
• Syk Kinase

Identifiers and source

Literature Corpus work

7d40d8bc-89c1-5778-96b7-41f4570bb4bf

DOI

10.1101/2022.04.24.489292