Article
Site-saturation mutagenesis: a powerful tool for structure-based design of combinatorial mutation libraries.
Current protocols in protein science - 1 Feb 2011
Chronopoulou Evangelia G, Labrou Nikolaos E
Abstract excerpt
This unit describes a method for site-saturation mutagenesis (SSM) using PCR amplification with degenerate synthetic oligonucleotides as primers. SSM allows the substitution of predetermined protein sites against all twenty possible amino acids at once. Therefore, SSM is a powerful approach in protein engineering to characterize structure-function relationships, as well as to create improved protein variants. The...
Topics
- Combinatorial Chemistry Techniques
- Gene Library
- Mutagenesis, Site-Directed
- Mutation
- Polymerase Chain Reaction
- Protein Engineering