Article

STRAIGHT-IN: A platform for high-throughput targeting of large DNA payloads into human pluripotent stem cells

2021-12-09

Abstract excerpt

<h4>SUMMARY</h4> Inserting large DNA payloads (>10 kb) into specific genomic sites of mammalian cells remains challenging. Applications ranging from synthetic biology to evaluating the pathogenicity of disease-associated variants for precision medicine initiatives would greatly benefit from tools that facilitate this process. Here, we merge the strengths of different classes of site-specific recombinases and comb...

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Literature Corpus work: 7d2948b9-8675-5bab-a99f-95ae3b6141a9
DOI: 10.1101/2021.12.08.471715

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