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Article

CRaTER enrichment for on-target gene editing enables generation of variant libraries in hiPSCs.

Journal of molecular and cellular cardiology - 1 Jun 2023

Friedman Clayton E, Fayer Shawn, Pendyala Sriram, Chien Wei-Ming, Loiben Alexander, Tran Linda, Chao Leslie S, Mckinstry Ashley, Ahmed Dania, Karbassi Elaheh, Fenix Aidan M, Murry Charles E, Starita Lea M, Fowler Douglas M, Yang Kai-Chun

Abstract excerpt

Standard transgenic cell line generation requires screening 100-1000s of colonies to isolate correctly edited cells. We describe CRISPRa On-Target Editing Retrieval (CRaTER) which enriches for cells with on-target knock-in of a cDNA-fluorescent reporter transgene by transient activation of the targeted locus followed by flow sorting to recover edited cells. We show CRaTER recovers rare cells with heterozygous,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 37019277
DOI: 10.1016/j.yjmcc.2023.03.017
PMCID: PMC10208587

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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