Article

CRaTER enrichment for on-target gene-editing enables generation of variant libraries in hiPSCs

2023-01-26

Abstract excerpt

<h4>SUMMARY</h4> Standard transgenic cell line generation requires screening 100-1000s of colonies to isolate correctly edited cells. We describe CR ISPR a On- T arget E diting R etrieval (CRaTER) which enriches for cells with on-target knock-in of a cDNA-fluorescent reporter transgene by transient activation of the targeted locus followed by flow sorting to recover edited cells. We show CRaTER recovers rare...

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Literature Corpus work: 725e1a2b-8728-56ee-b475-a8187a1ea662
DOI: 10.1101/2023.01.25.525582

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