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Article

Microhomology-assisted scarless genome editing in human iPSCs.

Nature communications - 5 Mar 2018

Kim Shin-Il, Matsumoto Tomoko, Kagawa Harunobu, Nakamura Michiko, Hirohata Ryoko, Ueno Ayano, Ohishi Maki, Sakuma Tetsushi, Soga Tomoyoshi, Yamamoto Takashi, Woltjen Knut

Abstract excerpt

Gene-edited induced pluripotent stem cells (iPSCs) provide relevant isogenic human disease models in patient-specific or healthy genetic backgrounds. Towards this end, gene targeting using antibiotic selection along with engineered point mutations remains a reliable method to enrich edited cells. Nevertheless, integrated selection markers obstruct scarless transgene-free gene editing. Here, we present a method...

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Share to Amino Acid Sequence
Share to Chromosomes, Human

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 29507284
DOI: 10.1038/s41467-018-03044-y
PMCID: PMC5838097

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APRT
HPRT1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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