Article
Harmonizing amplification and cleavage kinetics through crRNA scaffold mutation enables robust one-pot CRISPR-Cas12 diagnostics.
Proceedings of the National Academy of Sciences of the United States of America - 15 Sept 2026
Jiang Qingyang, Xu Ruiquan, Lin Zhixin, Sun Chenyu, Ye Feidi, Ye Yanyan, Lin Duoming, Li Zhen, Mai Jingwen, Lin Zhihong, Qu Jiuxin, Deng Xiaoyan, Ge Chenchen, Wang Dou
Abstract excerpt
CRISPR-based diagnostics (CRISPR-Dx) integrated with isothermal nucleic acid amplification have emerged as a promising strategy for point-of-care molecular testing. Their practical deployment, however, remains constrained by workflow-related limitations. Conventional two-step formats are laborious and highly susceptible to aerosol contamination during amplicon transfer, whereas simplified one-pot formats often...
Read the complete abstract on PubMedTopics
- CRISPR-Cas Systems
- Kinetics
- Humans
- Nucleic Acid Amplification Techniques
- SARS-CoV-2