Article

Differential efficacies of Cas nucleases on microsatellites involved in human disorders and associated off-target mutations

2019-11-29

Abstract excerpt

Microsatellite expansions are the cause of more than 20 neurological or developmental human disorders. Shortening expanded repeats using specific DNA endonucleases may be envisioned as a gene editing approach. Here, a new assay was developed to test several CRISPR-Cas nucleases on microsatellites involved in human diseases, by measuring at the same time double-strand break rates, DNA end resection and homologous r...

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Literature Corpus work: 7c77ac6f-fd1b-5287-b237-5b244074da65
DOI: 10.1101/857714

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