Article

Highly enhanced transgene integration in human pluripotent stem cells by serine integrase BxbI

2024-10-19

Abstract excerpt

<h4>Summary</h4> Genome engineering and especially integration of large DNA payloads in human pluripotent stem cells (hPSC) remains challenging largely due to the sensitive nature of hPSCs. Site specific recombinases such as the serine integrase Bxb1 have been utilised successfully to target hPSC, however the targeting efficiencies remain low. Here we leveraged codon optimisation, inclusion of a nuclear localisat...

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Literature Corpus work: 7c4bb46c-8e75-50be-b0b1-e421cf09bd39
DOI: 10.1101/2024.10.18.619063

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