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Article

Baculoviral transduction facilitates TALEN-mediated targeted transgene integration and Cre/LoxP cassette exchange in human-induced pluripotent stem cells.

Nucleic acids research - 1 Oct 2013

Zhu Haibao, Lau Cia-Hin, Goh Sal-Lee, Liang Qingle, Chen Can, Du Shouhui, Phang Rui-Zhe, Tay Felix Chang, Tan Wee-Kiat, Li Zhendong, Tay Johan Chin-Kang, Fan Weimin, Wang Shu

Abstract excerpt

Safety and reliability of transgene integration in human genome continue to pose challenges for stem cell-based gene therapy. Here, we report a baculovirus-transcription activator-like effector nuclease system for AAVS1 locus-directed homologous recombination in human induced pluripotent stem cells (iPSCs). This viral system, when optimized in human U87 cells, provided a targeted integration efficiency of 95.21%...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 23945944
DOI: 10.1093/nar/gkt721
PMCID: PMC3799456

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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