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Prevention of Transgene Silencing During Human Pluripotent Stem Cell Differentiation

2025-04-11

Abstract excerpt

<h4>SUMMARY</h4> While high and stable transgene expression can be achieved in undifferentiated pluripotent stem cells, conventional transgene expression systems are often silenced upon differentiation. Silencing occurs with both randomly integrated transgenes, introduced via transposase or lentiviral methods, and with transgenes targeted to specific genomic sites, including at commonly used safe harbor loci. The...

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Literature Corpus work
0a26f220-16ad-55bc-90fa-9b12092a5470
DOI
10.1101/2025.04.07.647695
Open publication

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Prevention of Transgene Silencing During Human Pluripotent Stem Cell DifferentiationDOI 10.1101/2025.04.07.647695
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