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Stable Transgene Expression in Primitive Human CD34 <sup>+</sup> Hematopoietic Stem/Progenitor Cells, Using the <i>Sleeping Beauty</i> Transposon System

18 Aug 2009

Abstract excerpt

Sleeping Beauty (SB) transposon-mediated integration has been shown to achieve long-term transgene expression in a wide range of host cells. In this study, we improved the SB transposon-mediated gene transfer system for transduction of human CD34(+) stem/progenitor cells by two approaches: (1) to increase the transposition efficacy, a hyperactive mutant of SB, HSB, was used; (2) to improve the expression of the...

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PMID: 19689196
DOI: 10.1089/hum.2009.109

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