Article

Transcriptome-wide profiling of alternative splicing regulators with CRISPore-seq

2025-11-25

Abstract excerpt

Alternative splicing creates diverse RNA isoforms from individual genes, yet single-cell CRISPR screens are limited to gene-level quantification and cannot detect changes in alternative splicing and transcript isoforms. To overcome this limitation, we develop CRISPore-seq, which couples massively-parallel CRISPR perturbations with joint short- and long-read transcriptomics. CRISPore-seq simultaneously captures gen...

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Literature Corpus work: 56807e76-317c-55f2-89b9-79916f488617
DOI: 10.1101/2025.11.25.690515

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