Article
A genome-wide atlas of human cell morphology.
Nature methods - 1 Mar 2025
Ramezani Meraj, Weisbart Erin, Bauman Julia, Singh Avtar, Yong John, Lozada Maria, Way Gregory P, Kavari Sanam L, Diaz Celeste, Leardini Eddy, Jetley Gunjan, Pagnotta Jenlu, Haghighi Marzieh, Batista Thiago M, Pérez-Schindler Joaquín, Claussnitzer Melina, Singh Shantanu, Cimini Beth A, Blainey Paul C, Carpenter Anne E, Jan Calvin H, Neal James T
Abstract excerpt
A key challenge of the modern genomics era is developing empirical data-driven representations of gene function. Here we present the first unbiased morphology-based genome-wide perturbation atlas in human cells, containing three genome-wide genotype-phenotype maps comprising CRISPR-Cas9-based knockouts of >20,000 genes in >30 million cells. Our optical pooled cell profiling platform (PERISCOPE) combines a...
Read the complete abstract on PubMedTopics
- Humans
- Genome, Human
- CRISPR-Cas Systems
- Phenotype
- Genomics