Article

Cytosine base editing workflow for quality-controlled multiplex-knockout hiPSC lines

2026-06-04

Abstract excerpt

<h4>SUMMARY</h4> Dissecting polygenic disease mechanisms requires human cell models that harbour multiple targeted genetic modifications in a defined background. However, generating and rigorously validating such models remains difficult. We developed a cytosine base editing workflow to generate multiplex-knockout (KO) human induced pluripotent stem cell (hiPSC) lines. First, we assessed six cytosine base editor...

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Literature Corpus work: 4f378224-f8e9-53f8-b320-395b3e855ec9
DOI: 10.64898/2026.06.02.729559

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