Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

High-throughput screening of human genetic variants by pooled prime editing

22 Mar 2025

Abstract excerpt

Multiplexed assays of variant effect (MAVEs) enable scalable functional assessment of human genetic variants. However, established MAVEs are limited by exogenous expression of variants or constraints of genome editing. Here, we introduce a pooled prime editing (PE) platform to scalably assay variants in their endogenous context. We first improve efficiency of PE in HAP1 cells, defining optimal prime editing guide...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR and Genetic Engineering
Share to Cytomegalovirus and herpesvirus research
Share to Virus-based gene therapy research

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 40120586
DOI: 10.1016/j.xgen.2025.100814

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

High-throughput screening of human genetic variants by pooled prime editingPMID 40120586Read ↗

High-throughput screening of human genetic variants by pooled prime editingSemantic proximity Read ↗

Editing stem cell genomes at scale to measure variant effects in diverse cell and genetic contextsSemantic proximity Read ↗

Massively parallel assessment of human variants with base editor screens.Semantic proximity Read ↗

Generating long deletions across the genome with pooled paired prime editing screensSemantic proximity Read ↗

High throughput PRIME editing screens identify functional DNA variants in the human genomeSemantic proximity Read ↗

Prime Editing Driven Functional Genomics: Bridging Genotype to Phenotype in the Post-Genomic Era.Semantic proximity Read ↗

Analyzing the functional effects of DNA variants with gene editing.Semantic proximity Read ↗

Massively parallel assessment of human variants with base editor screensSemantic proximity Read ↗

Decoding disease-relevant variants with base and prime editors at scale.Semantic proximity Read ↗

Prime editor-based high-throughput screening reveals functional synonymous mutations in human cells.Semantic proximity Read ↗

A Pangenomic Method for Establishing a Somatic Variant Detection Resource in HapMap MixturesSemantic proximity Read ↗

High-throughput discovery of rare insertions and deletions in large cohorts.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.