Article
Argonaute integrated single-tube PCR system enables supersensitive detection of rare mutations.
Nucleic acids research - 21 Jul 2021
Liu Qian, Guo Xiang, Xun Guanhua, Li Zhonglei, Chong Yuesheng, Yang Litao, Wang Hongxia, Zhang Fengchun, Luo Shukun, Cui Li, Zhao Pengshu, Ye Xingyu, Xu Heshan, Lu Hui, Li Xiao, Deng Zixin, Li Kai, Feng Yan
Abstract excerpt
Technological advances in rare DNA mutations detection have revolutionized the diagnosis and monitoring of tumors, but they are still limited by the lack of supersensitive and high-coverage procedures for identifying low-abundance mutations. Here, we describe a single-tube, multiplex PCR-based system, A-Star, that involves a hyperthermophilic Argonaute from Pyrococcus furiosus (PfAgo) for highly efficient...
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