Article

Highly specific enrichment of rare nucleic acids using <i>Thermus thermophilus</i> Argonaute

2018-12-10

Abstract excerpt

<h4>ABSTRACT</h4> Characterization of disease-associated, cell-free nucleic acids (liquid biopsy) provides a powerful, minimally-invasive means for early detection, genotyping, and personalized therapy; but is challenged by alleles of interest differing by single nucleotide from and residing among large abundance of wild-type alleles. We describe a new multiplexed enrichment assay, dubbed NAVIGATER, that utilizes...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 18bcbf29-41d0-5816-8c14-6df1bdbc949c
DOI: 10.1101/491738

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Highly specific enrichment of rare nucleic acids using <i>Thermus thermophilus</i> ArgonauteDOI 10.1101/491738Read

Highly specific enrichment of rare nucleic acid fractions using Thermus thermophilus argonaute with applications in cancer diagnostics.Semantic proximity Read

Argonaute integrated single-tube PCR system enables supersensitive detection of rare mutations.Semantic proximity Read

Elimination of unaltered DNA in mixed clinical samples via nuclease-assisted minor-allele enrichment.Semantic proximity Read

Single-nucleotide variant profiling in liquid biopsy with RECO-Cas.Semantic proximity Read

Nuclease-Assisted, Multiplexed Minor-Allele Enrichment: Application in Liquid Biopsy of Cancer.Semantic proximity Read

Temperature-boosted PAM-less activation of CRISPR-Cas12a combined with selective inhibitors enhances detection of SNVs with VAFs below 0.01.Semantic proximity Read

Enrichment of short mutant cell-free DNA fragments enhanced detection of pancreatic cancerSemantic proximity Read

Depletion of Abundant Sequences by Hybridization (DASH): Using Cas9 to remove unwanted high-abundance species in sequencing libraries and molecular counting applicationsSemantic proximity Read

Detection of low-frequency mutations in clinical samples by increasing mutation abundance via the excision of wild-type sequences.Semantic proximity Read

Method development for pancreatic and ovarian cancer baseline ctDNA detection and measurable residual disease monitoringSemantic proximity Read

Enrichment of short mutant cell-free DNA fragments enhanced detection of pancreatic cancerSemantic proximity Read

Multiplexed Elimination of Wild-Type DNA and High-Resolution Melting Prior to Targeted Resequencing of Liquid Biopsies.Semantic proximity Read