Article
Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV.
Talanta - 15 May 2021
Wang Longyu, He Ruyi, Lv Bin, Yu Xiao, Liu Yang, Yang Jun, Li Wenqiang, Wang Yuan, Zhang Hang, Yan Guangbo, Mao Wuxiang, Liu Linlin, Wang Fei, Ma Lixin
Abstract excerpt
Infectious diseases caused by viruses such as SARS-CoV-2 and HPV have greatly endangered human health. The nucleic acid detection is essential for the early diagnosis of diseases. Here, we propose a method called PLCR (PfAgo coupled with modified Ligase Chain Reaction for nucleic acid detection) which utilizes PfAgo to only use DNA guides longer than 14-mer to specifically cleave DNA and LCR to precisely...
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