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Repurposing Type I-A CRISPR-Cas3 for a robust diagnosis of human papillomavirus (HPV)

13 Jul 2024

Abstract excerpt

R-loop-triggered collateral single-stranded DNA (ssDNA) nuclease activity within Class 1 Type I CRISPR-Cas systems holds immense potential for nucleic acid detection. However, the hyperactive ssDNase activity of Cas3 introduces unwanted noise and false-positive results. In this study, we identified a novel Type I-A Cas3 variant derived from Thermococcus siculi, which remains in an auto-inhibited state until it is...

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PMID: 39003402
DOI: 10.1038/s42003-024-06537-3

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