Article

A simplified hybrid capture approach retains high specificity and enables PCR-free workflow

2025-03-19

Abstract excerpt

Hybrid capture is a critical technology for selective enrichment of genomic regions of interest, enabling cost-effective focused sequencing in both clinical and research applications. We present a simplified hybrid capture approach that eliminates complexities typically associated with hybridization-based selection methods by directly loading the hybridization product onto the sequencing flow cell. The workflow re...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 6e897e93-be5d-55de-b2aa-21cb8ed45aad
DOI: 10.1101/2025.03.18.644033

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A simplified hybrid capture approach retains high specificity and enables PCR-free workflowDOI 10.1101/2025.03.18.644033Read

The next generation of target capture technologies - large DNA fragment enrichment and sequencing determines regional genomic variation of high complexity.Semantic proximity Read

Sequencing small genomic targets with high efficiency and extreme accuracy.Semantic proximity Read

DNA enrichment by allele-specific hybridization (DEASH): a novel method for haplotyping and for detecting low-frequency base substitutional variants and recombinant DNA molecules.Semantic proximity Read

dCATCH-Seq: improved sequencing of large continuous genomic targets with double-hybridization.Semantic proximity Read

Population-based rare variant detection via pooled exome or custom hybridization capture with or without individual indexing.Semantic proximity Read

Targeted nanopore sequencing with Cas9-guided adapter ligation.Semantic proximity Read

A programmable method for massively parallel targeted sequencing.Semantic proximity Read

Comparison of custom capture for targeted next-generation DNA sequencing.Semantic proximity Read

Evaluation of Hybridization Capture Versus Amplicon-Based Methods for Whole-Exome Sequencing.Semantic proximity Read

Comparison of three targeted enrichment strategies on the SOLiD sequencing platform.Semantic proximity Read

Assessment of Capture and Amplicon-Based Approaches for the Development of a Targeted Next-Generation Sequencing Pipeline to Personalize Lymphoma Management.Semantic proximity Read

A novel fast hybrid capture sequencing method for high-efficiency common human coronavirus whole-genome acquisitionSemantic proximity Read